This grant supports the expansion of a major $9 million, multi-institutional research project funded by the National Institute of Health. The NIH study aims to determine the bacterial community composition of 340 paired house dust and infant stool samples in a case cohort epidemiological study. Sloan funds will enable the research team to expand their analysis to not just bacteria, but fungi. Led Dr. Susan Lynch of the University of California, San Francisco, the research team will perform high resolution fungal community profiling of the 340 paired samples, conduct a variety of statistical analyses to determine whether relationships exist between fungal and bacterial community composition in house dust and infant stool, and use multivariate regression analysis to relate fungal/bacterial house dust microbiome composition to measure house characteristics and allergic disease outcomes to identify key factors that influence the home and infant stool microbiome and are related to human health status.